NAME
    Alien::SeqAlignment::hmmer3 - find, build and install the hmmer3 tools

SYNOPSIS
    To execute the hmmer3 set of tools, you can use the following code:

     use Alien::SeqAlignment::hmmer3;
     use Env qw( @PATH );
     unshift @PATH, Alien::SeqAlignment::hmmer3->bin_dir;

    Now you can run the hmmer3 tools as:

    HMMER3 SUITE

      system Alien::SeqAlignment::hmmer3->alimask       (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmalign      (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmbuild      (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmconvert    (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmemit       (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmfetch      (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmlogo       (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmpgmd       (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmpgmd_shard (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmpress      (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmsearch     (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmsim        (parameters & options);
      system Alien::SeqAlignment::hmmer3->hmmstat       (parameters & options);
      system Alien::SeqAlignment::hmmer3->jackhmmer     (parameters & options);
      system Alien::SeqAlignment::hmmer3->makehmmerdb   (parameters & options);
      system Alien::SeqAlignment::hmmer3->nhmmer        (parameters & options);
      system Alien::SeqAlignment::hmmer3->nhmmscan      (parameters & options);
      system Alien::SeqAlignment::hmmer3->phmmer        (parameters & options);

    EASEL SUITE

      system Alien::SeqAlignment::hmmer3->esl_afetch     (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alimanip   (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alimap     (parameters & options);
      system Alien::SeqAlignment::hmmer3->asl_alimask    (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alimerge   (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alipid     (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alirev     (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_alistat    (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_compalign  (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_compstruct (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_construct  (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_histplot   (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_mask       (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_mixdchlet  (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_reformat   (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_selectn    (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_seqrange   (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_seqstat    (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_sfetch     (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_shuffle    (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_ssdraw     (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_translate  (parameters & options);
      system Alien::SeqAlignment::hmmer3->esl_weight     (parameters & options);

DESCRIPTION
    This distribution provides HMMER3 so that it can be used by other Perl
    distributions that are on CPAN. The source code will be downloaded from
    the HMMER3 website, if the HMMER3 is not found in the system path. The
    url used is : http://eddylab.org/software/hmmer/hmmer.tar.gz The program
    will then be built and installed in a private share location. The build
    provides the various CLI tools in the hmmer3 AND Easel suites. Note that
    the Easel tools are also provided by the HMMER3 suite and this Alien
    assumes that Easel has been previously installed with HMMER3 during a
    prior system install. If this is not the case, attempt to use the Easel
    files will terminate any Perl programs that try to use the Easel tools
    from within this distribution.

METHODS : HMMER3 SUITE
  alimask
      Alien::SeqAlignment::hmmer3->alimask  (parameters & options);
    alimask - add column mask to a multiple sequence alignment

  hmmalign
      Alien::SeqAlignment::hmmer3->hmmalign (parameters & options);
    hmmalign - make multiple sequence alignment using a profile

  hmmbuild
      Alien::SeqAlignment::hmmer3->hmmbuild (parameters & options);
    hmmbuild - build profile from input multiple alignment

  hmmconvert
      Alien::SeqAlignment::hmmer3->hmmconvert (parameters & options);
    hmmconvert - convert between different profile file formats

  hmmemit
      Alien::SeqAlignment::hmmer3->hmmemit (parameters & options);
    hmmemit - generate (sample) sequences from a profile

  hmmfetch
      Alien::SeqAlignment::hmmer3->hmmfetch (parameters & options);
    hmmfetch - retrieve profile(s) from a profile file

  hmmlogo
      Alien::SeqAlignment::hmmer3->hmmlogo (parameters & options);
    hmmlogo - produce a conservation logo graphic from a profile

  hmmpgmd
      Alien::SeqAlignment::hmmer3->hmmpgmd (parameters & options);
    hmmpgmd - search daemon for the hmmer.org website

  hmmpgmd_shard
      Alien::SeqAlignment::hmmer3->hmmpgmd_shard (parameters & options);
    hmmpgmd_shard - sharded search daemon for the hmmer.org website

  hmmscan
      Alien::SeqAlignment::hmmer3->hmmscan (parameters & options);
    hmmscan - search sequence(s) against a profile database

  hmmsearch
      Alien::SeqAlignment::hmmer3->hmmsearch (parameters & options);  
    hmmsearch - search profile against sequence database

  hmmpress
      Alien::SeqAlignment::hmmer3->hmmpress (parameters & options);
    hmmpress - prepare profile database for hmmscan

  phmmer
      Alien::SeqAlignment::hmmer3->phmmer (parameters & options);
    phmmer - search single sequence against sequence database

  jackhmmer
      Alien::SeqAlignment::hmmer3->jackhmmer (parameters & options);
    jackhmmer - iteratively search single sequence against database

  nhmmer
      Alien::SeqAlignment::hmmer3->nhmmer (parameters & options);
    nhmmer - search DNA query against DNA sequence database

  nhmmscan
      Alien::SeqAlignment::hmmer3->nhmmscan (parameters & options);
    nhmmscan - search DNA sequence against a DNA profile database

  hmmstat
      Alien::SeqAlignment::hmmer3->hmmstat (parameters & options);
    hmmstat - show summary statistics for a profile file

  makehmmerdb
      Alien::SeqAlignment::hmmer3->makehmmerdb (parameters & options);
    makehmmerdb - prepare an nhmmer binary database

  hmmsim
      Alien::SeqAlignment::hmmer3->hmmsim (parameters & options);
    hmmsim - collect score distributions on random sequences

METHODS : EASEL SUITE
    Note that the names of the methods are the same as the functions in the
    CLI of the EASEL suite, but with the "-" replaced by "_", e.g.
    esl-afetch becomes esl_afetch.

  esl_afetch
      Alien::SeqAlignment::hmmer3->esl_afetch (parameters & options);
    esl_afetch - retrieve alignments from a multi-MSA database

  esl_alimanip
      Alien::SeqAlignment::hmmer3->esl_alimanip (parameters & options);
    esl_alimanip - manipulate a multiple sequence alignment

  esl_alimap
      Alien::SeqAlignment::hmmer3->esl_alimap (parameters & options);
    esl_alimap - map two alignments to each other

  esl_alimask
      Alien::SeqAlignment::hmmer3->esl_alimask (parameters & options);

  esl_alimerge
        Alien::SeqAlignment::hmmer3->esl_alimerge (parameters & options);
    esl_alimerge - merge alignments based on their reference (RF) annotation

  esl_alipid
      Alien::SeqAlignment::hmmer3->esl_alipid (parameters & options);
    esl_alipid - calculate pairwise percent identities for all sequence

  esl_alirev
      Alien::SeqAlignment::hmmer3->esl_alirev (parameters & options);
    esl_alirev - reverse complement a multiple alignment

  esl_alistat
      Alien::SeqAlignment::hmmer3->esl_alistat (parameters & options);
    esl_alistat - summarize a multiple sequence alignment file

  esl_compalign
      Alien::SeqAlignment::hmmer3->esl_compalign (parameters & options);
    esl_compalign - compare two multiple sequence alignments

  esl_compstruct
      Alien::SeqAlignment::hmmer3->esl_compstruct (parameters & options);
    esl_compstruct - calculate accuracy of RNA secondary structure predictions

  esl_construct
      Alien::SeqAlignment::hmmer3->esl_construct (parameters & options);
    esl_construct - describe or create a consensus secondary structure

  esl_histplot
      Alien::SeqAlignment::hmmer3->esl_histplot (parameters & options);
    esl_histplot - collate data histogram, output xmgrace datafile

  esl_mask
      Alien::SeqAlignment::hmmer3->esl_mask (parameters & options);
    esl_mask - mask sequence residues with Xs (or other characters)

  esl_mixdchlet
      Alien::SeqAlignment::hmmer3->esl_mixdchlet (parameters & options);
    esl_mixdchlet - fitting mixture Dirichlets to count data

  esl_reformat
      Alien::SeqAlignment::hmmer3->esl_reformat (parameters & options);
    esl_reformat - convert sequence file formats

  esl_selectn
      Alien::SeqAlignment::hmmer3->esl_selectn (parameters & options);
    esl_selectn - select random subset of lines from file

  esl_seqrange
      Alien::SeqAlignment::hmmer3->esl_seqrange (parameters & options);
    esl_seqrange - determine a range of sequences for one of many parallel

  esl_seqstat
        Alien::SeqAlignment::hmmer3->esl_seqstat (parameters & options);
    esl_seqstat - summarize contents of a sequence file

  esl_sfetch
      Alien::SeqAlignment::hmmer3->esl_sfetch (parameters & options);
    esl_sfetch - retrieve (sub-)sequences from a sequence file

  esl_shuffle
      Alien::SeqAlignment::hmmer3->esl_shuffle (parameters & options);
    esl_shuffle - shuffling sequences or generating random ones

  esl_ssdraw
      Alien::SeqAlignment::hmmer3->esl_ssdraw (parameters & options);
    esl_ssdraw - create postscript secondary structure diagrams

  esl_translate
      Alien::SeqAlignment::hmmer3->esl_translate (parameters & options);
    esl_translate - translate DNA sequence in six frames into individual

  esl_weight
      Alien::SeqAlignment::hmmer3->esl_weight (parameters & options);
    esl_weight - calculate sequence weights in MSA(s)

SEE ALSO
    *   HMMER <http://hmmer.org/>

        HMMER: biosequence analysis using profile hidden Markov models

        HMMER is used for searching sequence databases for sequence
        homologs, and for making sequence alignments. It implements methods
        using probabilistic models called profile hidden Markov models
        (profile HMMs).

        HMMER is often used together with a profile database, such as Pfam
        or many of the databases that participate in Interpro. But HMMER can
        also work with query sequences, not just profiles, just like BLAST.
        For example, you can search a protein query sequence against a
        database with phmmer, or do an iterative search with jackhmmer.

        HMMER is designed to detect remote homologs as sensitively as
        possible, relying on the strength of its underlying probability
        models. In the past, this strength came at significant computational
        expense, but as of the new HMMER3 project, HMMER is now essentially
        as fast as BLAST.

        HMMER can be downloaded and installed as a command line tool on your
        own hardware, and now it is also more widely accessible to the
        scientific community via new search servers at the European
        Bioinformatics Institute.

    *   Alien

        Documentation on the Alien concept itself.

    *   Alien::Base <https://metacpan.org/pod/Alien::Base>

        The base class for this Alien. The methods in that class allow you
        to use the static and the dynamic edlib library in your code.

    *   Alien::Build::Manual::AlienUser
        <https://metacpan.org/dist/Alien-Build/view/lib/Alien/Build/Manual/A
        lienUser.pod>

        Detailed manual for users of Alien classes.

AUTHOR
    Christos Argyropoulos <chrisarg@gmail.com>

COPYRIGHT AND LICENSE
    This software is copyright (c) 2024 by Christos Argyropoulos.

    This is free software; you can redistribute it and/or modify it under
    the same terms as the Perl 5 programming language system itself.